Section 9.1 - Introduction to Protein Detection

Figure 9.1 Growth of proteomics. Using the search parameter “proteom*” the NCBI database was queried for citations. The number of citations in each year plotted against the year shows the tremendous increase in research and interest in the proteomics field. Figure 9.2 2-D protein gels of tumor vs. normal cells stained for glycoproteins and total proteins. Lysates from rat liver tumor cells (top panels) or rat normal liver cells (bottom panels) were run on identical 2-D gels. Following electrophoresis, the gels were stained with the Pro-Q Emerald 300 glycoprotein detection reagent (left panels) (available in the Pro-Q Emerald 300 Kits P-21855 and P-21857). After documentation of the fluorescence signal, the gel was stained with the SYPRO Ruby protein gel stain (right panels) (S-12000, S-12001, S-21900). Proteomics — A Rapidly Developing Field